HyperChrom AR rProtein A/G Agarose 4FF Agarose
HyperChrom AR rProtein A/G Agarose 4FF Agarose is an affinity chromatography medium for antibody purification, separated by specific interaction between antigens and antibodies, mainly used for the purification of IgG in culture media, serum, ascites or hybridoma cell culture supernatant, or the purification of recombinant proteins containing Fc fragments.
The HyperChrom AR rProtein A FF Agarose and HyperChrom Protein G 4FF Agarose in this product are covalently attached to 4% high crosslinked, high flow rate agarose and mixed in a 1:1 ratio. Each milliliter of HyperChrom AR rProtein A/G Agarose 4FF Agoros can bind approximately 20 mg of human IgG.
HyperChrom AR rProtein A/G Agarose 4FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
rProtein A/G (E. coli recombinant expression, 1:1). |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Dynamic load |
~20 mg human IgG/mL chromatography medium |
|
Flow rates are recommended |
50-400 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability |
3-9 |
| Components | PC2007-25 mL | PC2007-100 mL |
| HyperChrom AR rProtein A/G Agarose 4FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||