HyperChrom AR rProtein A FF Agarose
HyperChrom AR rProtein A FF Agarose is an affinity chromatography medium for antibody purification, separated by specific interaction between antigen and antibody, mainly used to purify monoclonal and polyclonal antibodies.
HyperChrom AR rProtein A FF Agarose has high selectivity, high yield, and easy to scale up properties: (1) recombinant protein A is produced in E. coli and engineered for directional conjugation to enhance binding load; Epoxy-based conjugation ensures low ligand shedding; recombinant protein A has a specificity similar to native protein A to the LgG Fc region, which improves selectivity and yield. (2) Process amplification is relatively simple.
HyperChrom AR rProtein A FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
rProtein A (E. coli recombinant expression) |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ligand density |
6 mg rProtein A/ml chromatography medium |
|
Dynamic load |
~35 mg human IgG/mL chromatography medium* |
|
Flow rates are recommended |
150-500 cm/h |
|
Maximum flow rate |
700 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
3-10 |
|
Chemical resistance |
6 M guanidine hydrochloride, 8 M urea, 2% benzyl alcohol, 75% ethanol, 1 mM NaOH (pH11), 0.1 M sodium citrate/HCl (pH3). |
|
** Dynamic load measurement conditions: column loading height: 5 cm, retention time 3 min, test buffer: 0.02 M NaH2PO4 solution, pH 7.0, test sample: human lgG sample, lgG sample per unit media volume (ml) when the penetration of lgG reaches 10%. **After 7 days of storage of chromatography medium at 40°C and pH 3-10, its physicochemical properties and functions did not change significantly. ***30% and 75% are v/v, volume ratio. |
|
| Components | PC2005-25 mL | PC2005-100 mL |
| HyperChrom AR rProtein A FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||