GSH/GSSG Ratio Assay Kit (Fluorometric)

Glutathione is a tripeptide composed of glutamic acid (Glu), cysteine (Cys), and glycine (Gly). Glutathione is mainly found in cells in two forms, namely reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG). Reduced glutathione is highly reducible and is the main active form in cells (accounting for >90%). Oxidized glutathione is formed by the dehydrogenation of two GSH. When the level of oxidative stress in cells increases, the content of oxidized glutathione rises, causing the GSH/GSSG ratio to decrease. Thus, the GSH/GSSG ratio serves as a key indicator of cellular oxidative stress.

This kit uses Thiolite Green to quantify the GSH/GSSG ratio. Thiolite Green can react with GSH and produce an intense green fluorescence (Ex/Em=504/522 nm) to quantify the GSH content in the sample. At the same time, this kit provides a GSSG Probe, which can reduce GSSG to GSH to detect the total GSH content. The GSH/GSSG ratio can be calculated after obtaining GSH and Total GSH content. For detecting GSH only, this kit is enough to be used for 200 assays.

Figure 1. Typical Total GSH and reduced GSH standard calibration curve