Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit

Glucose-6-phosphate dehydrogenase (G6PDH) is the key rate-limiting enzyme of the pentose phosphate pathway. It catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconolactone, simultaneously reducing NADP⁺ to NADPH. NADPH is essential for maintaining cellular redox homeostasis (e.g., glutathione regeneration) and supporting biosynthetic processes such as fatty acid synthesis. G6PDH deficiency leads to insufficient NADPH production, which can result in various conditions, including neonatal jaundice and hemolytic anemia. Therefore, accurate measurement of G6PDH activity is of great significance for the diagnosis, research, and prevention of related disorders.

This kit is designed for the quantitative determination of G6PDH activity in samples such as cells, tissues, and serum. The principle is based on the catalytic conversion of G6P to 6-PG by G6PDH, during which NADP⁺ is reduced to NADPH. Subsequently, in the presence of the electron carrier 1-mPMS, NADPH reduces the WST-8 reagent to produce a water-soluble orange-yellow formazan dye. The amount of formazan generated, which is proportional to the G6PDH activity in the sample, can be measured by its characteristic absorbance at 450 nm. This kit features high sensitivity, simple operation, and reliable performance, making it highly suitable for the rapid and accurate analysis of G6PDH activity.

Figure 1. Typical standard curve and assay data. Typical standard curve (left), determination of G6PDH levels in different samples (right).