Glucose-6-phosphate (G6P) Colorimetric Assay Kit

Glucose-6-phosphate (G6P) is a key intermediate in glucose metabolism, involved in both the glycolytic pathway and as the initiating substrate of the pentose phosphate pathway. It plays a key role in cellular energy supply and biosynthesis. The detection of G6P levels is of great significance for studying the regulation of cellular energy metabolism, glucose metabolism, and related diseases (such as diabetes mellitus and metabolic syndrome).

The Glucose-6-phosphate (G6P) Colorimetric Assay Kit is based on a WST-8 chromogenic reaction for detecting G6P content in cells, tissues, or other samples. Glucose-6-phosphate dehydrogenase (G6PDH) oxidizes G6P and reduces NADP⁺ to NADPH. The generated NADPH can reduce WST-8 to orange-yellow formazan under the action of electron cocoupler 1-mPMS. Formazan has a maximum absorption peak at 450 nm, and its absorbance is directly proportional to the G6P level in the sample. The kit offers high detection sensitivity and a wide linear range, showing a good linear relationship between 0–500 μM.

Figure 1. Typical standard curve and assay data. (A) The reference standard curve, (B) The G6P concentration in different samples generated using this kit.