Fluoxetine HCl
Fluoxetine HCl (CAS 56296-78-7) is a selective serotonin reuptake inhibitor (SSRI) that specifically blocks presynaptic serotonin (5-HT) transporters, resulting in elevated extracellular serotonin levels. It modulates serotonergic signaling through inhibition of serotonin-induced membrane currents in cloned 5HT2C receptors, exhibiting an IC50 around 20 μM, and binds 5HT2C receptors expressed in HeLa cells (Ki approximately 65-97 nM). Fluoxetine treatment has been shown in vivo to stimulate neurogenesis and enhance neuronal maturation and synaptic plasticity within rat hippocampus and prefrontal cortex. Widely employed in neuroscience research, fluoxetine aids in studies of depression, stress resilience, and neuro-regulatory mechanisms involving serotonergic signaling.
| Storage | Store at -20°C |
| M.Wt | 345.79 |
| Cas No. | 56296-78-7 |
| Formula | C17H18F3NO·HCl |
| Solubility | insoluble in H2O; ≥17.3 mg/mL in DMSO; ≥32.2 mg/mL in EtOH |
| Chemical Name | N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CNCCC(C1=CC=CC=C1)OC2=CC=C(C=C2)C(F)(F)F.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
Xenopus oocytes expressing 5HT2C receptor or 5HT receptors |
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Preparation method |
The solubility of this compound in DMSO is >17.3 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
100 μM |
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Applications |
Fluoxetine inhibited the membrane currents elicited by serotonin (5HT) in Xenopus oocytes expressing either cloned 5HT2c receptors or 5HT receptors. Responses of 5HT2c receptors, elicited by nM concentrations of 5HT, were rapidly and reversibly blocked by micromolar concentrations of fluoxetine. In accord with the electrophysiological results, fluoxetine inhibited the binding of [3H]5HT to 5HT2c receptors, and the binding to 5HT receptors in rat cortex membranes was also inhibited but less efficiently. |
| Animal experiment [2]: | |
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Animal models |
Male Sprague-Dawley rats |
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Dosage form |
5 mg/kg/day, i.p. |
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Application |
Olanzapine or fluoxetine treatment also increased the number of proliferating cells in the prelimbic cortex. In contrast, there was no effect of either drug in the subventricular zone or primary motor cortex. Subchronic (7 days) administration of olanzapine had no effect on cell proliferation in hippocampus or prelimbic cortex, consistent with the time course for the effect of fluoxetine and the therapeutic actions of antidepressant treatment. The combination of olanzapine plus fluoxetine did not result in a greater induction of cell proliferation in either brain region. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Ni Y G, Miledi R. Blockage of 5HT2C serotonin receptors by fluoxetine (Prozac)[J]. Proceedings of the National Academy of Sciences, 1997, 94(5): 2036-2040. [2] Kodama M, Fujioka T, Duman R S. Chronic olanzapine or fluoxetine administration increases cell proliferation in hippocampus and prefrontal cortex of adult rat[J]. Biological psychiatry, 2004, 56(8): 570-580. |
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Quality Control & MSDS
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Chemical structure
