EZ Cap™ Nano Luciferase mRNA
Nano Luciferase (NLuc) is an engineered novel luciferase derived from deep-sea shrimp. With a molecular weight of only 19.1 kDa, it is much smaller than traditional firefly luciferase (Fluc) and Renilla luciferase (Rluc). NLuc emits light in an ATP-independent manner. It catalyzes the oxidation of the specific substrate furimazine to produce intense blue luminescence at approximately 460 nm. Its brightness is over 100-fold higher than that of conventional luciferases. Featuring excellent stability, NLuc serves as a highly sensitive bioluminescent reporter gene. It is widely applicable to gene regulation and functional research, live-cell dynamic monitoring, and in vivo imaging experiments.
Key features:
Cap1 Structure: The mRNA incorporates a Cap1 analog at the 5' end, ensuring high translation initiation efficiency, improved stability, and reduced innate immune recognition, leading to stronger and more sustained luciferase expression.
poly(A) tail: It contains an optimized poly(A) tail of approximately 100 nucleotides. This specific length is engineered to maximize transcript stability by resisting degradation and to synergize with the 5' cap for superior, sustained translation efficiency, ensuring robust protein yield in your applications.
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mRNA Length |
856 nucleotides |
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Concentration |
~1 mg/mL |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
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General tips |
Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. |
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Shipping Condition |
ship with dry ice |
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Related Biological Data
