Ellagic Acid hydrate

Ellagic Acid hydrate (CAS No.: 314041-08-2) is a plant-derived polyphenolic metabolite, typically produced by the hydrolysis of ellagitannins in various fruits and nuts, and is widely used as a small-molecule chemical probe in redox biology and kinase signaling research; in biochemical systems, it potently inhibits protein kinase CK2 through competitive binding to the ATP site, with reported IC50 of approximately 40 nM and Ki of approximately 20 nM, and shows weaker inhibitory activity against Lyn and protein kinase A (low micromolar level), demonstrating preference for CK2, and is therefore commonly used to dissect signaling programs related to CK2-dependent phosphorylation, including cell cycle regulation, survival pathways, DNA damage response, and transcriptional regulation; its intrinsic antioxidant capacity also supports its application in studies of oxidative stress mechanisms and redox-sensitive targets; its in vitro activity generally falls within the nanomolar to micromolar range, with specific effects depending on the enzyme or cellular context studied; in practice, it is commonly used for enzymatic profiling, target validation, and mechanistic studies, as well as exploratory screening in early discovery to evaluate pathway occupancy signals, with common experimental settings including biochemical enzyme activity systems and cultured mammalian cell models; the specific concentration or dose used in experiments depends on the study design and objective, and its use is limited to laboratory research purposes.