Drofenine Hydrochloride

Drofenine Hydrochloride (CAS No.: 548-66-3) is a synthetic small molecule commonly classified as a neuropharmacology and ion channel focused research tool, with established relevance to cholinesterase biology, membrane excitability, and neuroinflammatory signaling studies in biomedical discovery settings. Reported mechanistic data indicate that it functions as a competitive butyrylcholinesterase inhibitor with activity in the low micromolar range and also modulates ion handling through inhibition of Kv2.1 associated potassium efflux and activation of TRPV3 linked calcium influx, supporting its use in multiparametric assays that interrogate cholinergic regulation, channel dependent stress responses, and cell state transitions. In cellular systems, this compound has been associated with modulation of Kv2.1 JNK NF kB and IkBa NF kB signaling axes, downregulation of Kv2.1 transcript and protein expression, and attenuation of oligomeric amyloid beta triggered microglial NLRP3 inflammasome activation together with reduced neuronal tau hyperphosphorylation related readouts, while additional observations in keratinocyte contexts indicate calcium entry effects and context dependent cytotoxicity that should be considered during assay design and concentration selection. As a blood brain barrier permeable spasmolytic legacy scaffold with anticholinergic functional background, it is broadly positioned for mechanism of action characterization, target deconvolution, phenotypic screening, and translationally oriented preclinical model research in neurodegeneration, peripheral nerve dysfunction, and smooth muscle biology, where the concentrations or doses used in experiments typically depend on specific experimental designs and research objectives.