dodecanedioate

dodecanedioate (CAS No.: 693-23-2) is a straight-chain dicarboxylate metabolite that functions as a small-molecule modulator of intermediary metabolism, positioned at the interface of lipid and carbohydrate oxidation pathways. It serves as an alternative energy substrate that can be rapidly utilized through mitochondrial β-oxidation, thereby influencing cellular energy homeostasis and metabolic flux without directly stimulating insulin-dependent signaling. Experimental studies indicate that dodecanedioate can interfere with key enzymatic steps and regulatory nodes involved in fatty acid and glucose metabolism, supporting its role in probing metabolic pathway integration and substrate utilization. In vitro and in vivo investigations suggest activity within low micromolar to millimolar concentration ranges, depending on cell type and metabolic context, with observed effects on energy expenditure, substrate preference, and metabolic signaling cascades. This compound is widely applied in biochemical and cell-based assays to investigate mitochondrial function, metabolic flexibility, and the regulation of energy balance, as well as in animal models examining systemic metabolic adaptations. The concentrations or doses used in experiments typically depend on specific experimental designs and research objectives, making dodecanedioate a valuable tool for studying metabolic regulation and pathway crosstalk in drug discovery and molecular biology research.