Disuccinimidyl Glutarate

Disuccinimidyl Glutarate (CAS No.: 79642-50-5) is a homobifunctional, amine-reactive crosslinking reagent widely utilized in biochemical and biomedical research for stable conjugation of proteins, peptides, and other biomolecules. Characterized by the presence of two reactive N-hydroxysuccinimide ester groups, it efficiently targets primary amines on lysine residues or N-termini, enabling the formation of robust, non-cleavable covalent linkages that support long-term structural and functional studies of macromolecular complexes. DSG is membrane-permeable, facilitating intracellular crosslinking applications, and is frequently employed in combination with fixation strategies such as formaldehyde treatment to enhance the detection and stabilization of protein–protein and protein–DNA interactions in chromatin and transcriptional studies. In drug discovery and bioconjugation research, DSG has been applied in the development of antibody–drug conjugates and other targeted delivery systems by linking antibodies to small-molecule payloads, thereby supporting selective targeting and controlled payload presentation under defined conditions. Although it does not act through classical receptor-mediated signaling pathways or exhibit direct pharmacological activity, its functional mechanism is based on covalent bond formation with accessible amine groups, and its effective use typically falls within low micromolar to millimolar concentration ranges depending on substrate availability and experimental design. DSG is broadly applied in in vitro systems including cell lysates and cultured cells, as well as in structural biology, proteomics, and chemical biology workflows, where reagent concentration and exposure conditions are optimized according to specific experimental objectives.