D-Fructose-6-phosphate (sodium salt)

D-Fructose-6-phosphate (sodium salt) (CAS No.: 26177-86-6) is an endogenous phosphorylated hexose that serves as a central metabolic intermediate in carbohydrate metabolism, functioning at the intersection of glycolysis, gluconeogenesis, and the pentose phosphate pathway. It is generated from fructose-1,6-bisphosphate via fructose-1,6-bisphosphatase and is reversibly isomerized to glucose-6-phosphate by phosphoglucose isomerase, representing a key regulatory node in the Embden–Meyerhof pathway. This metabolite participates in cellular energy homeostasis and biosynthetic processes, and is also implicated in the modulation of cell growth and autophagic responses. In biochemical research, D-fructose-6-phosphate is widely utilized as a substrate for enzymatic assays, including studies of glucosamine-6-phosphate synthase and transaldolase activities, and as a probe to investigate metabolic flux and pathway regulation in vitro and in cellular systems. Additionally, it has been applied in mechanistic studies of neurodegenerative disease models, including investigations related to Lewy body pathology. As a naturally occurring metabolite rather than a direct pharmacological inhibitor or activator, its measurable effects in experimental systems are typically observed in concentration ranges from micromolar to millimolar levels, depending on assay design and cellular context, and dosing regimens should be optimized according to specific experimental objectives.