D-Fructose 1,6-bisphosphate (sodium salt hydrate)

D-Fructose 1,6-bisphosphate (sodium salt hydrate) (CAS No.: 81028-91-3) is a phosphorylated carbohydrate intermediate that plays a central role in cellular energy metabolism, functioning at a key regulatory junction within glycolysis and gluconeogenesis. Endogenously generated from fructose-6-phosphate by phosphofructokinase, it is subsequently cleaved into triose phosphates during glycolysis, while its hydrolysis by fructose-1,6-bisphosphatase constitutes a rate-limiting step in gluconeogenesis, thereby positioning it as a critical metabolic node controlling carbon flux. This compound is widely used in biochemical and metabolic research to investigate enzyme kinetics and regulatory mechanisms involving phosphofructokinase and fructose-1,6-bisphosphatase, as well as broader signaling pathways governing cellular bioenergetics. In cancer biology, it serves as a valuable tool for probing the metabolic reprogramming of tumor cells, particularly their reliance on aerobic glycolysis, and for evaluating therapeutic strategies targeting metabolic vulnerabilities. Although it does not act as a conventional inhibitor or agonist with defined potency metrics, its biochemical effects are typically studied across micromolar to millimolar concentration ranges depending on assay conditions. D-Fructose 1,6-bisphosphate (sodium salt hydrate) is commonly applied in cell-based assays, enzymatic studies, and metabolic flux analyses, with experimental concentrations tailored to specific model systems and research objectives in drug discovery and systems biology.