D-2-Aminobutyric acid

D-2-Aminobutyric acid (CAS No.: 2623-91-8) is a chiral non-proteinogenic amino acid belonging to the class of D-alpha-amino acids, characterized by the D-configuration at the alpha-carbon, and is widely utilized as a biochemical tool in studies of amino acid metabolism and stereospecific enzymatic processes. It is recognized as a substrate of D-amino acid oxidase, an enzyme involved in the oxidative deamination of D-amino acids, thereby serving as a useful probe for investigating enzyme kinetics, substrate specificity, and the regulation of D-amino acid catabolism in vitro and in cellular systems. Through its interaction with D-amino acid oxidase, D-2-aminobutyric acid contributes to the generation of corresponding keto acids and hydrogen peroxide, linking it to studies of oxidative stress and redox biology under controlled experimental conditions. Although detailed potency metrics are not consistently reported, its enzymatic turnover is typically examined within micromolar to millimolar concentration ranges depending on assay sensitivity and system design. This compound is commonly applied in biochemical assays, enzymology research, and metabolic pathway characterization, as well as in the development of screening platforms for modulators of D-amino acid oxidase activity. Experimental usage in cell-based or enzyme-based models varies according to the specific research objectives, with concentrations adjusted to achieve measurable enzymatic or metabolic responses without introducing non-specific effects.