Cytidine 5'-triphosphate (sodium salt)

Cytidine 5'-triphosphate (sodium salt) (CAS No.: 36051-68-0) is an endogenous nucleoside triphosphate that plays a central role in cellular nucleotide metabolism and serves as a key substrate in the biosynthesis of nucleic acids and phospholipids. As an essential intermediate in the de novo pyrimidine biosynthetic pathway, it is generated from uridine 5′-triphosphate via cytidine triphosphate synthase and participates in DNA and RNA synthesis through regulation of polymerase-mediated nucleotide incorporation and associated enzymatic processes. In addition to its metabolic functions, this molecule has been reported to act as an agonist of certain cation-permeable ligand-gated ion channels, supporting its involvement in nucleotide-mediated signaling pathways. In vitro studies typically employ cytidine 5'-triphosphate in concentration ranges spanning the nanomolar to micromolar scale, depending on assay conditions and biological context. This compound is widely utilized in biochemical and molecular biology research to investigate nucleotide metabolism, enzymatic regulation, and pyrimidine pathway dynamics, as well as in cell-based and organismal models to explore mechanisms underlying nucleotide-associated disorders and to support target identification and validation in drug discovery workflows, with experimental concentrations tailored to specific study designs.