Cyclic AMP
Cyclic adenosine 3’, 5’-monophosphate (cAMP) was the first second messenger to be identified, with the IC50 of LPA- or EGF-stimulated ERK1 was 0.1-0.2 Mm?[1].
Ras is a common target for growth factors including epidermal growth factor (EGF) and lysophosphatidic acid (LPA), which activate the Raf-MEK-ERK pathway. Raf is likely to be a link between Ras and ERK cascade, of which Raf-1activates MEK in vitro.
Quiescent Rat1 cells were incubated with 1 Mm?dibutyryl cAMP for 10 min before stimulation with LPA or EGF, ERK activation was completely inhibited by protein immunoblots. The?ability of the phorbol ester PMA to activate MAP kinase was also inhibited by dibutyryl cAMP [1].
Reference:
[1] Cook S J, McCormick F. Inhibition by cAMP of Ras-Dependent?Activation of Raf?[J]. Science. 1993, 262(5136):1069-1072.
| Physical Appearance | A solid |
| Storage | -20°C |
| M.Wt | 329.21 |
| Cas No. | 60-92-4 |
| Formula | C10H12N5O6P |
| Synonyms | Adenosine 3',5'-cyclic monophosphate; cAMP; Cyclic adenosine monophosphate; NSC 94017; NSC 143670 |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥16.45 mg/mL in H2O |
| Chemical Name | (4aR,6R,7R,7aS)-6-(6-amino-9H-purin-9-yl)-2,7-dihydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinine 2-oxide |
| Canonical SMILES | C=CCSSSCC=C |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |