Cy5 Boc-hydrazide (non-sulfonated)
Catalog No.
A8144
carbonyl-reactive dye
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Boc-protected Cy5 hydrazide is a reactive dye for the labeling of aldehydes and ketones with high aqueous solubility. Trifluoroacetic acid or other strong acid can remove the Boc-(tert-butoxycarbonyl) protective group. Reactive hydrazide can couple with various carbonyl groups yielding Cy5-labeled molecules. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| M.Wt | 633.26 |
| Formula | C37H49ClN4O3 |
| Solubility | good in dichloromethane, polar organic solvents (DMF, DMSO), alcohols, low in water |
| Chemical Name | 1-(6-(2-(tert-butoxycarbonyl)hydrazinyl)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium |
| SDF | Download SDF |
| Canonical SMILES | CC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(NNC(OC(C)(C)C)=O)=O)C4=CC=CC=C4C3(C)C |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 646 | Emission max (nm) | 662 |
| Extinction Coefficient (M-1cm-1) | 250000 | Quantum Yield | 0.2 |
| CF260 | 0.03 | CF280 | 0.04 |
Quality Control & MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
