Creatinine Colorimetric/Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Creatinine is a product of creatine phosphate and is produced at a constant rate. Serum creatinine is used to determine glomerular filtration rate (GFR), which is an indicator of kidney function. Only when nephron is significantly damaged (>75%), blood creatinine levels will increase. Creatinine clearance is used to standardize excretion of other compounds such as isoprostanes.
The Creatinine Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for accurate detection of creatinine levels in various biological fluids (serum, urine or CSF) based on colorimetric and fluorometric method. In the assay, creatinine is converted to creatine by creatininase, then creatine is converted to sarcosine, which is subsequently and specifically oxidized to generate a product which reacts with Creatinine Probe to yield fluorescence (Ex/Em = 538/587 nm) and red color (λmax = 570 nm). The kit is also suited for measurement of creatinine in serum/plasma, as well as in urine and other biological samples.
Features & Properties
|Features||Fast and convenient; Simple procedure; Takes approx. 1 hour; Kit contains all necessary reagents for accurate measurement of creatinine.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||Creatinine Assay Buffer, Creatinine Probe, Creatinase, Creatininase, Creatinine Enzyme Mix, Creatinine (10 µmol)|