Concanavalin A (Con A)-FITC
Concanavalin A (Con A), derived from Canavalia ensiformis (Concanavalin), is a plant lectin protein (Mw = 104 kDa), each subunit can bind one Ca2+ and one Mn2+, contains a sugar-binding site, and when the metal ions bind to Con A, it can bind various glycoproteins, glycolipids and α-D-glucose and a-D-mannose moieties in sugars, while FITC is a fluorescent dye that fluoresces green (Ex/Em: 495 nm/515 nm) under ultraviolet light. This combination allows Con A-FITC to have a high visualization capability for the detection of cell surface carbohydrate molecules. Concanavalin A-FITC (Con A-FITC) is a fluorescently labeled protein that combines Concanavalin A and fluorescein isothiocyanate (FITC) and is widely used in biological research, especially in the field of flow cytometry.
This product is FITC-labeled concanavalin A, which is in solution form, which can be used in immunofluorescence and glycobiology research, such as as being used as a probe to detect carbohydrate molecules on the cell surface, because of its ability to bind to a variety of sugars, it can bind to the cell surface expressing a variety of sugars, and can be detected and analyzed by flow cytometry. It is used for immunohistochemical staining to observe the distribution of sugars in tissues or cells by fluorescence microscopy.