ChIP Assay Kit (Protein A/G Magnetic Beads)

ChIP (chromatin immunoprecipitation) is an experimental technique used to study protein-DNA interactions, allowing researchers to identify binding sites in the genome of specific proteins, typically transcription factors or histone modifications. The basic steps of a ChIP experiment include cross-linking, lysis, immunoprecipitation, reverse fixation, DNA extraction, and DNA analysis. Common analysis methods include magnetic bead method and agarose method, the conventional agarose method requires long-term centrifugation, while the magnetic bead method does not need centrifugation, only needs a short time of magnetic separation, which can effectively avoid the mechanical force effect caused by centrifugation on the immune complex, and fully ensure the natural state of the immune complex.

Protein A/G Magnetic Beads are used to immunoprecipitate a wide range of antibodies, including mouse IgG1, IgG2a, IgG2b, IgG3, IgA, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit IgG, rabbit and goat polyclonal Abs, as well as human IgG1, IgG2, IgG3 and IgG4. At the same time, the non-specific binding of Protein A+G Agarose presaturated with Salmon Sperm DNA and the target genomic DNA is greatly reduced. If the ChIP product is used for chromatin immunoprecipitation sequencing (ChIP-Seq), possible Salmon Sperm DNA data needs to be removed from the data.

Pre-mixed control primers (Control Primers) are provided for amplification of some of the corresponding sequences of human GAPDH: 5'-TACTAGCGGTTTTACGGGCG-3' and 5'-TCGAACAGGAGGAGCAGAGAGCGA-3'.

The ChIP Assay Kit can be used for routine chromatin immunoprecipitation, resulting in a total of 22 samples.