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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Celastramycin A is an antibiotic that inhibits the growth of bacteria and mycobacteria and is also a potent innate immune suppressor [1][2].
Celastramycin A is a benzoyl pyrrole-type compound isolated from endophytic bacteria Streptomyces MaB-QuH-8 living in plants of the Celastraceae family. Celastramycin A exhibited high activity against a series of multiresistent bacteria and mycobacteria [1]. Celastramycin A inhibited the growth of Gram-positive bacteria and mycobacteria with minimal inhibitory concentration (MIC) as low as 0.05 μg/ml [1]. In the ex vivo Drosophila culture system, Celastramycin A showed a potent immunosuppressive effect with IC50 value of 0.008 μg/mL. In human umbilical vein endothelial cells (HUVECs), Celastramycin A potently inhibited the production of IL-8 with IC50 value of 0.06 μg/mL. So Celastramycin A could be used as a lead compound for novel immunosuppressive agents [2].
References:[1]. Pullen C, Schmitz P, Meurer K, et al. New and bioactive compounds from Streptomyces strains residing in the wood of Celastraceae. Planta. 2002 Nov;216(1):162-7.[2]. Kikuchi H, Sekiya M, Katou Y, et al. Revised structure and synthesis of celastramycin a, a potent innate immune suppressor. Org Lett. 2009 Apr 16;11(8):1693-5.
Cell lines
Human umbilical vein endothelial cells (HUVECs)
Preparation method
The solubility of this compound in DMSO is ≤ 20mg/ml. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
0.06 μg/ml
Applications
In human umbilical vein endothelial cells (HUVEC), celastramycin A effectively inhibited IL-8 production with an IC50 value of 0.06 μg/mL. Celastramycin A therefore can be used as a lead compound for novel immunosuppressants.
References:
[1]. Kikuchi H, Sekiya M, Katou Y, Ueda K, Kabeya T, Kurata S, Oshima Y. Revised structure and synthesis of celastramycin a, a potent innate immune suppressor. Org Lett. 2009 Apr 16;11(8):1693-5. doi: 10.1021/ol9002306. PMID: 19317429.