Caspase-6 Colorimetric Assay Kit
Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-6 is a member of the caspase family. Caspase-6 is processed by caspases 7, 8 and 10, and can also undergo self-processing. Caspase-6 acts as a downstream enzyme in the caspase activation cascade and interacts with Caspase 8.
Caspase-6 Colorimetric Assay Kit provides a convenient and simple way for detecting the VEID-dependent caspase activity. When cleavage of VEID from the labeled substrate VEID-p-nitroaniline (VEID-pNA) by Caspase-6 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-6 activity.
| Features | Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
| Kit components | Cell Lysis Buffer 2X Reaction Buffer VEID-pNA (4 mM) DTT (1 M) |
| Components | K2015-25 25 assays |
K2015-100 100 assays |
K2015-200 200 assays |
K2015-400 400 assays |
Part Number |
| Cell Lysis Buffer | 25 ml | 100 ml | 100 ml | 100 ml | K2015-C-1 |
| 2 X Reaction Buffer | 2 ml | 4 x 2 ml | 16 ml | 32 ml | K2015-C-2 |
| VEID-pNA (4 mM) | 125 μl | 500 μl | 2 x 0.5 ml | 2 x 1 ml | K2015-C-3 |
| DTT (1 M) | 100 μl | 400 μl | 400 μl | 400 μl | K2015-C-4 |
Store at -20°C. | |||||