Calcein AM (Calcein acetoxymethyl ester)
Calcein AM (calcein acetoxymethyl ester) is a non-fluorescent, uncharged molecule, which is freely permeable to the plasma membrane of viable cells. Upon entering live cells, calcein AM is cleaved by nonspecific esterases in the cytoplasm to form membrane-impermeant calcein, a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively. Calcein AM has wide-ranging applications in the fields of cell viability, cell-cell communication and cytotoxicity. In addition, calcein is a substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3), which makes calcein AM a useful tool for studying these proteins and their regulation.
References:
1. Miles FL, Lynch JE, Sikes RA. Cell-based assays using calcein acetoxymethyl ester show variation in fluorescence with treatment conditions. Journal of Biological Methods, 2015, 2(3): e29.
2. Liu X, Zhu XY, Chen M, et al. Resveratrol protects PC12 cells against OGD/R-induced apoptosis via the mitochondrial-mediated signaling pathway. Acta Biochimica et Biophysica Sinica, 2016, 48(4): 342-353.
3. Polli JW, Wring SA, Humphreys JE, et al. Rational use of in vitro P-glycoprotein assays in drug discovery. Journal of Pharmacology and Experimental Therapeutics, 2001, 299(2): 620-628.
4. Tiberghien F, Loor F. Ranking of P-glycoprotein substrates and inhibitors by a calcein-AM fluorometry screening assay. Anticancer Drugs, 1996, 7(5): 568-578.
5. Szakács G, Jakab K, Antal F, et al. Diagnostics of multidrug resistance in cancer. Pathology & Oncology Research, 1998, 4(4): 251-257.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 994.86 |
| Cas No. | 148504-34-1 |
| Formula | C46H46N2O23 |
| Solubility | Soluble in DMSO |
| Chemical Name | tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate |
| SDF | Download SDF |
| Canonical SMILES | O=C1O[C@@]2(C3=CC=CC=C31)C(C=C4)=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4OC(C)=O)OC5=C2C=CC(OC(C)=O)=C5CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
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Purity ≥90.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
