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Calcein AM (Calcein acetoxymethyl ester)

Catalog No.
B7755
A cell-permeable dye, converted to its fluorescent form after entering live cells
Grouped product items
SizePriceStock Qty
1mg
$206.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

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Email: [email protected]

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Background

Calcein AM (Calcein acetoxymethyl ester; CAS: 148504-34-1) is a non-fluorescent, cell-permeable derivative of calcein employed for viability assays and live-cell fluorescence staining. Upon entering live cells, intracellular esterases hydrolyze Calcein AM to release highly fluorescent calcein, a charged molecule unable to diffuse across intact membranes, resulting in intracellular retention and pronounced green fluorescence emission. Its negligible cytotoxicity and insensitivity to pH variations support broad use in mammalian viability assays, typically combined with propidium iodide (PI) for differentiating living versus compromised cells.

Product Citation

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt994.86
Cas No.148504-34-1
FormulaC46H46N2O23
SolubilitySoluble in DMSO
Chemical Nametetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate
SDFDownload SDF
Canonical SMILESO=C1O[C@@]2(C3=CC=CC=C31)C(C=C4)=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4OC(C)=O)OC5=C2C=CC(OC(C)=O)=C5CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Quality Control & MSDS

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Chemical structure

Calcein AM (Calcein acetoxymethyl ester)