Calcein AM (Calcein acetoxymethyl ester)
Catalog No.
B7755
A cell-permeable dye, converted to its fluorescent form after entering live cells
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Calcein AM (Calcein acetoxymethyl ester; CAS: 148504-34-1) is a non-fluorescent, cell-permeable derivative of calcein employed for viability assays and live-cell fluorescence staining. Upon entering live cells, intracellular esterases hydrolyze Calcein AM to release highly fluorescent calcein, a charged molecule unable to diffuse across intact membranes, resulting in intracellular retention and pronounced green fluorescence emission. Its negligible cytotoxicity and insensitivity to pH variations support broad use in mammalian viability assays, typically combined with propidium iodide (PI) for differentiating living versus compromised cells.
- 1. Jiamin Qin, Guojuan Fan, et al. "Dynamic Covalent Bond-Based Nanoassembly of Curcumin to Enhance the Selective Photothermal Therapy for Tumor Treatment." Int J Nanomedicine. 2025 Mar 30:20:3861-3875 PMID: 40181832
- 2. Yin Ni, Bang-Chuan Hu, et al. "Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI." Theranostics. 2021 Sep 13;11(19):9431-9451. PMID: 34646379
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 994.86 |
| Cas No. | 148504-34-1 |
| Formula | C46H46N2O23 |
| Solubility | Soluble in DMSO |
| Chemical Name | tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate |
| SDF | Download SDF |
| Canonical SMILES | O=C1O[C@@]2(C3=CC=CC=C31)C(C=C4)=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4OC(C)=O)OC5=C2C=CC(OC(C)=O)=C5CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
-
Purity ≥90.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
