BSA-Palmitate Saturated Fatty Acid Complex (5 mM)

BSA-Palmitate Saturated Fatty Acid Complex is composed of palmitic acid and fatty acid-free bovine serum albumin (BSA) at an approximate molar ratio of 6:1 (palmitate:BSA). This complex has been widely utilized for efficient fatty acid delivery to cultured cells, facilitating the study of fatty acid oxidation and related metabolic processes in various cellular models[1-3]. BSA and BSA-fatty acid complexes are suitable for use in short-term cell culture applications (acute treatments up to 18 hours). However, for long-term applications (exceeding 25 hours), it is recommended that the product be filter-sterilized using a 0.2 μm filter and a sterile receptacle; this procedure does not compromise the performance of the complex. For optimal results, it is advisable to use this product in conjunction with a BSA control when working with BSA-fatty acid complexes (5 mM).

References:

[1] Alsabeeh N, Chausse B, Kakimoto PA, Kowaltowski AJ, Shirihai O. Cell culture models of fatty acid overload: Problems and solutions. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Feb;1863(2):143-151. doi: 10.1016/j.bbalip.2017.11.006. Epub 2017 Nov 15. PMID: 29155055; PMCID: PMC5969525.

[2] Wang D, Green MF, McDonnell E, Hirschey MD. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol Biol. 2013;1077:241-58. doi: 10.1007/978-1-62703-637-5_16. PMID: 24014411; PMCID: PMC3817486.

[3] Bentebibel A, Sebastián D, Herrero L, López-Viñas E, Serra D, Asins G, Gómez-Puertas P, Hegardt FG. Novel effect of C75 on carnitine palmitoyltransferase I activity and palmitate oxidation. Biochemistry. 2006 Apr 11;45(14):4339-50. doi: 10.1021/bi052186q. PMID: 16584169.