Bacterial Biofilm Matrix Stain

Biofilms present significant challenges for fluorescent labeling and subsequent imaging due to their complex architecture. These microbial communities exhibit heterogeneous thickness across surfaces, creating barriers to stain penetration. Within biofilms, bacterial cells exist in diverse physiological states influenced by varying microenvironmental conditions. Additionally, biofilms contain numerous undefined components, such as the extracellular polymeric matrix, whose composition varies with species and growth conditions.
Bacterial Biofilm Matrix Stain is designed for labeling biofilms. This stain effectively labels a broad spectrum of protein classes, including glycoproteins, phosphoproteins, lipoproteins, calcium-binding proteins, and fibrillar proteins. Bacterial Biofilm Matrix Stain exhibits excitation and emission maxima at 450 nm and 610 nm, producing a red fluorescence signal. It has been validated for staining the matrix of Pseudomonas aeruginosa and certain E. coli strains. The product is a valuable tool for comprehensive biofilm analysis.