Cy3.5 maleimide (non-sulfonated)
Catalog No.
A8264
Labeling of cysteine residues, as well as other thiolated molecules
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Cy3.5 maleimide can be used for selective labeling of free sulfhydryl groups (e.g. cysteine residues in peptides, proteins and oligonucleotides). Before labeling, cystines should be prepared with reductant such as TCEP (tris-carboxyethylphosphine). The mono-reactive malemides is particularly good for the labeling of antibody, that’s because the dye molecules are located away from the antibody binding site and functioning of the labeled molecules can be protected.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 715.32 |
| Cas No. | N/A |
| Formula | C44H47ClN4O3 |
| Solubility | ≥71.5 mg/mL in DMSO; ≥28.5 mg/mL in EtOH with ultrasonic |
| Chemical Name | 3-(6-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E)-3-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium chloride |
| SDF | Download SDF |
| Canonical SMILES | CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C4=[N+](CCCCCC(NCCN5C(C=CC5=O)=O)=O)C6=CC=C(C=CC=C7)C7=C6C4(C)C.[Cl-] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 591 | Emission max (nm) | 604 |
| Extinction Coefficient (M-1cm-1) | 116000 | Quantum Yield | 0.35 |
| CF260 | 0.29 | CF280 | 0.22 |
Quality Control & MSDS
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Chemical structure
