Anti-His Tag Rabbit Polyclonal Antibody

The polyhistidine tag (His-Tag) is a short peptide motif in proteins consisting of at least five histidine (His) residues, with the most common form being the 6×His tag (sequence: HHHHHH), typically located at the N-terminus or C-terminus of recombinant proteins. Due to its small size and low immunogenicity, this tag enables efficient one-step purification via immobilized metal ion affinity chromatography (IMAC), and is particularly suitable for recombinant proteins expressed in prokaryotic systems. This rabbit polyclonal antibody exhibits high affinity and has been validated for Western Blot (WB) and ELISA, making it suitable for both qualitative and quantitative analysis of His-tagged fusion proteins.