Annexin V-EGFP Apoptosis Kit

Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V. Enhanced green fluorescent protein (EGFP) is a fluorescent reagent that is more brighter and more photo-stable. 

 Annexin V-EGFP Apoptosis Detection Kit uses an EGFP fusion of annexin V that can easily detect PS on the cell surface after initiating apoptosis. The one-step staining procedure needs only 10 minutes. The result can be analyzed by fluorescence microscopy or by flow cytometry with a FITC filter. The Annexin V-EGFP Apoptosis Detection Kit can differentiate apoptosis vs necrosis when performing both Annexin V-EGFP and PI staining. In addition, this kit can be directly performed on live cells.