Adipose tissue secretes various biologically active soluble factors that regulates metabolism of glucose and fatty acid. The Adiponectin (human) Elisa Assay Kit is an enzyme-linked immunosorbent assay for quantitative analysis of andiponectin in human serum, plasma and tissue/cell culture supernatants etc. In this assay, human adiponectin specific monoclonal antibody has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells. The adiponectin present is than attached to the immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors generated is proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
