Acid Yellow 36

Acid Yellow 36 (CAS No.: 587-98-4) is a synthetic azo dye, also known as Metanil Yellow, that is primarily classified as a research chemical colorimetric probe and acidic range pH indicator, with a well characterized transition from red at strongly acidic conditions near pH 1.2 to yellow near pH 2.3, making it useful for controlled assay environments that require rapid visual or spectrophotometric readouts of proton activity; in biomedical research settings it is generally positioned within analytical and mechanistic screening workflows rather than as a selective bioactive ligand, and literature level descriptions indicate functional relevance to xenobiotic response biology through reported induction of cytochrome P450 associated isoenzymes and cytosolic enzyme systems, consistent with a broad cellular stress and metabolic adaptation profile rather than a single high affinity receptor target, while specific pathway resolution and direct binding partners remain incompletely defined and are commonly interpreted in the context of enzyme regulation networks related to biotransformation; correspondingly, robust standardized potency metrics such as IC50, EC50, or MIC are not consistently established for this compound in the public research context, so activity is more appropriately described as context dependent and typically evaluated across screening concentrations spanning low micromolar to higher micromolar or millimolar exploratory ranges according to assay sensitivity and matrix effects; typical preclinical laboratory uses include in vitro buffer and cell associated assay calibration, exploratory studies of metabolism linked enzyme induction, and method development in multi parameter discovery platforms, with occasional translation into nonclinical experimental models for mechanistic toxicology or pharmacology support, where the concentrations or doses used in experiments typically depend on specific experimental designs and research objectives.