5-Methyltetrahydrofolic Acid

5-Methyltetrahydrofolic Acid (CAS No.: 134-35-0) is a biologically active, methylated derivative of tetrahydrofolate and represents the predominant circulating form of folate in plasma and cerebrospinal fluid, serving as a central cofactor in one-carbon metabolism and methylation reactions. As a key regulator of cellular biochemical processes, it participates in nucleotide biosynthesis, amino acid interconversion, and epigenetic modulation through methyl group donation, thereby influencing cellular proliferation and metabolic homeostasis. Experimental evidence indicates that 5-Methyltetrahydrofolic Acid modulates cardiovascular-related signaling pathways, including the upregulation of endothelin-1 production in low-density lipoprotein-treated endothelial cells, highlighting its relevance in studies of endothelial function and vascular biology. In vitro investigations typically examine its activity across nanomolar to micromolar concentration ranges, depending on the cellular context and assay design, particularly in endothelial, neuronal, and metabolic cell models. This compound is widely utilized in biochemical, pharmacological, and metabolic research, including studies of folate-dependent enzymatic pathways, redox balance, and disease-associated metabolic dysregulation, as well as in screening platforms aimed at elucidating mechanisms of cardiovascular and neurobiological processes.