4-PYRAZOLECARBOXYLIC ACID

4-PYRAZOLECARBOXYLIC ACID (CAS No.: 37718-11-9) is a naturally occurring pyrazole-derived metabolic intermediate that participates in heterocyclic compound-associated metabolic pathways and has been implicated in the regulation of purine and pyrimidine metabolism through modulation of enzyme activity and nucleotide homeostasis. Identified as a downstream metabolite of fomepizole, this compound has attracted attention in biochemical and pharmacological research for its role as a scaffold or intermediate in the development of inhibitors targeting AlkB homolog 1 (ALKBH1), a dioxygenase involved in nucleic acid demethylation and epigenetic regulation. Through its influence on metabolic networks and enzyme systems, 4-pyrazolecarboxylic acid is frequently employed in metabolomics studies to characterize endogenous metabolic alterations under disease-relevant conditions or in response to experimental perturbations, supporting the identification of pathway-level changes and potential biomarkers. In vitro investigations typically explore its effects within low micromolar to higher concentration ranges depending on assay design, particularly in studies examining nucleotide metabolism, enzyme activity modulation, or cancer-related cellular processes such as those modeled in gastric cancer research. Its application spans cell-based assays, biochemical pathway analysis, and early-stage drug discovery workflows, where experimental concentrations and conditions are selected according to specific study objectives and system requirements.