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BCA Protein Assay Kit

Catalog No.
K4101
Protein Quantification Assay Kit, BCA Method
Grouped product items
SizePriceStock Qty
200T
$50.00
In stock
500T
$70.00
In stock
1000T
$100.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

BCA, fully named Bicinchoninic Acid Assay, is a protein quantification method based on the biuret reaction principle. Under alkaline conditions, protein peptide bonds reduce Cu2+  (from copper sulfate) to Cu+ . Subsequently, BCA chelates Cu+  to form a purple-colored complex, which strongly absorbs light at a wavelength of 562 nm. The intensity of the colored complex is proportional to protein concentration, as the reduction of Cu2+  or the amount of Cu+  formed directly reflects the amount of protein present in the solution. Thus, protein concentration in an unknown sample can be determined by measuring absorbance.

This assay kit features easy operation, high stability, high sensitivity and excellent compatibility. It can detect protein concentrations as low as 25 μg/mL, with a minimum detection limit as low as 0.5 μg protein. It requires a sample volume of only 1–20 μL. The assay shows good linearity in the concentration range of 50–2000 μg/mL.

Product Citation

Quality Control

Quality Control & DataSheet

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Components and Storage

Components K4101-200 T K4101-500 T K4101-1000 T
Reagent A 40 mL 100 mL 2 X 100 mL
Reagent B 1.2 mL 3 mL 6 mL
Bovine serum albumin (BSA) 10 mg 15 mg 30 mg
BCA Protein Assay Reagent 6 mL 9 mL 18 mL

Store the components at 4℃ for a year.