ChIP Assay Kit (Protein A+G Agarose)
ChIP (chromatin immunoprecipitation) is an experimental technique used to study protein-DNA interactions. It allows researchers to identify binding sites in the genome for specific proteins, typically transcription factors or histone modifications. The basic steps of a ChIP experiment include cross-linking, lysis, immunoprecipitation, reverse fixation, DNA extraction, and DNA analysis.
Protein A+G Agarose is suitable for immunoprecipitation of a wide range of antibodies, including mouse IgG1, IgG2a, IgG2b, IgG3, IgA, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit IgG, rabbit and goat polyclonal Abs, as well as human IgG1, IgG2, IgG3 and IgG4. At the same time, the non-specific binding of Protein A+G Agarose presaturated with Salmon Sperm DNA and the target genomic DNA is greatly reduced. Pre-mixed control primers (Control Primers) are provided for amplification of some of the corresponding sequences of human GAPDH: 5'-TACTAGCGGTTTTACGGGCG-3' and 5'-TCGAACAGGAGGAGCAGAGAGCGA-3'.
The ChIP Assay Kit can be used for routine chromatin immunoprecipitation, resulting in a total of 22 samples.
| Components |
K4625-22 T |
|
Protein A+G Agarose/Salmon Sperm DNA |
3 mL |
|
Glycine Solution (10X) |
30 mL |
|
ChIP Dilution Buffer |
48 mL |
|
Low Salt Immune Complex Wash Buffer |
24 mL |
|
High Salt Immune Complex Wash Buffer |
24 mL |
|
LiCl Immune Complex Wash Buffer |
24 mL |
|
TE Buffer |
48 mL |
|
0.5 M EDTA |
250 μL |
|
5 M NaCl |
500 μL |
|
1 M Tris, pH 6.5 |
500 μL |
|
SDS Lysis Buffer |
10 mL |
|
Control Primers (5 μM each) |
0.1 mL |
|
Store all the components at 4℃ for 1 year. |
|