HyperTrap AR rProtein A/G 4FF Column
HyperTrap AR rProtein A/G 4FF Column is an affinity chromatography z-packed column for antibody purification that is separated by a specific interaction between antigen and antibody, mainly for the purification of IgG in culture fluid, serum, ascites, or hybridoma cell culture supernatant, or the purification of recombinant proteins containing Fc fragments.
HyperChrom AR rProtein A FF Agarose and HyperChrom Protein G 4FF Agarose in this product are covalently connected to 4% high crosslinked, high flow rate agarose and mixed in a 1:1 ratio. Each milliliter of HyperChrom AR rProtein A/G Agarose 4FF Agoros can bind approximately 20 mg of human IgG.
1. HyperTrap AR rProtein A/G 4FF Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
|
Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
|
Withstand pressure |
0.3 MPa |
0.3 MPa |
|
Workflow Speed* |
1 mL/min |
1-3 mL/min |
|
Operating temperature |
4 - 30°C |
4 - 30°C |
|
Storage condition |
Store the components at 4℃ for 5 years. |
|
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap AR rProtein A/G 4FF Column chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
rProtein A/G (E. coli recombinant expression, 1:1). |
|
Scaffolding |
Highly cross-linked agarose |
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Average particle size |
90 μm |
|
Dynamic load |
~20 mg human IgG/mL chromatography medium |
|
Flow rates are recommended |
50-400 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability |
3-9 |