HyperChrom Protein G 4FF Agarose
HyperChrom Protein G 4FF Agarose is an affinity chromatography medium made by coupling Protein G to the Chromstar substrate by CNBr activation. Protein G can bind to the Fc region of a variety of mammalian IgG and has a weak affinity for its Fab region.
Compared to Protein A, Protein G has a broader binding spectrum. Therefore, HyperChrom Protein G 4FF Agarose can be used to isolate and purify immunoglobulins and their fragments from different liquid samples such as cell culture media or serum. HyperChrom Protein G 4FF Agarose can also be used to isolate immune complexes.
HyperChrom Protein G 4FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
Protein G (E. coli recombinant expression). |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ligand density |
~2 mg Protein G/mL chromatography medium |
|
Dynamic load |
≥ 20 mg human IgG/mL chromatography medium* |
|
Flow rates are recommended |
90-300 cm/h |
|
Maximum flow rate |
400 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
3-10 |
|
Chemical resistance |
Common aqueous solution, 30% isopropanol*** 75% ethanol*** 6 M guanidine hydrochloride, 8 M urea |
|
*Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 200 cm/h, test buffer: 0.02 M NaH2PO4 solution, pH 7.0, test sample: 2 mg/m IgG sample, when the penetration of IgG reaches 10%, unit medium volume (mL) IgG loading volume (mg). **After 7 days of storage in the environment of 40°C and pH 3-9, the physicochemical properties and functions of the chromatography medium did not change significantly. *** 30% and 75% are the volume ratio (v/v). |
|
| Components | PC2006-25 mL | PC2006-100 mL |
| HyperChrom Protein G 4FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||