Micro BCA Protein Assay Kit
Bicinchoninic Acid Assay (BCA) is a protein quantification method based on the biuret reaction. Under alkaline conditions, protein peptide bonds reduce Cu2+ ions from copper sulfate to Cu+, subsequently forming a purple-colored complex by chelating Cu+ with BCA, which strongly absorbs at a wavelength of 562 nm. The color intensity of the chelate is correlated with protein concentration, while the reduction of Cu2+ to Cu+ is directly proportional to the amount of protein present in the solution. Thus, protein concentration can be determined by measuring the absorbance.
This kit is specifically designed to quantify low-concentration protein samples, measuring total protein concentrations in the range of 0.5-20 µg/mL. The kit features ease of operation, excellent stability, high sensitivity, and wide compatibility. It exhibits good compatibility with many ionic and non-ionic detergents.
| Components | K4102-500 T | K4102-2000 T |
| Reagent A | 40 mL | 160 mL |
| Reagent B | 38 mL | 150 mL |
| Reagent C | 2 mL | 6 mL |
| Bovine serum albumin (BSA) | 6 mg | 15 mg |
| BCA Protein Assay Reagent | 15 mL | 35 mL |
Store Reagent B at 4°C away from light and the other components at 4°C for a year. | ||