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Forskolin

Catalog No.
B1421
type I adenylate cyclase agonist
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$55.00
In stock
25mg
$70.00
In stock
50mg
$105.00
In stock
100mg
$150.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Forskolin (CAS 66575-29-9) is a diterpenoid isolated from the plant Coleus forskohlii, known to directly activate adenylate cyclase type I, thereby elevating cellular cyclic AMP (cAMP) concentrations. It exhibits an IC50 of approximately 41 nM against adenylate cyclase. By increasing intracellular cAMP, forskolin modulates signaling pathways involved in inflammation and oxidative stress, reducing macrophage activation and subsequent production of thromboxane B2 and superoxide. Forskolin has been explored in various experimental models and clinical contexts, including cardiovascular disorders, diabetes mellitus, and asthma research.

References:
[1].  Seamon KB, Padgett W, Daly JW. Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells. Proc Natl Acad Sci U S A, 1981, 78(6): 3363-3367.
[2].  Awad JA, Johnson RA, Jakobs KH, et al. Interactions of forskolin and adenylate cyclase. Effects on substrate kinetics and protection against inactivation by heat and N-ethylmaleimide. J Biol Chem, 1983, 258(5): 2960-2965.
[3].  Takeda J, Adachi K, Halprin KM, et al. Forskolin activates adenylate cyclase activity and inhibits mitosis in in vitro in pig epidermis. J Invest Dermatol, 1983, 81(3): 236-240.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt410.5
Cas No.66575-29-9
FormulaC22H34O7
Solubilityinsoluble in H2O; ≥13.43 mg/mL in EtOH; ≥20.53 mg/mL in DMSO
Chemical Name[(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-3-ethenyl-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-5,6,6a,8,9,10-hexahydro-2H-benzo[f]chromen-5-yl] acetate
SDFDownload SDF
Canonical SMILESC[C@]1([C@@H]([C@@H]2O)C(C)(C)CC[C@@H]1O)[C@]3(O)[C@]([C@H]2OC(C)=O)(C)O[C@@](C)(C=C)CC3=O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1, 2]:

Cell lines

Human mesenchymal stem cells

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0.075-0.2 mM for 4 days or 7 days; or 10 μM

Applications

Forskolin concentration-dependently decreased human mesenchymal stem cells proliferation after 4 days. Moreover, Forskolin increased alkaline phosphatase (ALP) expression of human mesenchymal stem cells in a dose-dependent manner. Additionally, Forskolin (10 μM) significantly stimulated both vasopressin and oxytocin release from the rat hypothalamo-neurohypophysial (H-NH) system.

Animal experiment [1]:

Animal models

Particles were implanted in subcutaneous pockets of nude male mice model

Dosage form

0.10 or 0.15mM Forskolin

Applications

Treatment with 0.10 mM Forskolin enhanced bone formation by human mesenchymal stromal cells in vivo.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

1. Doorn, J., Siddappa, R., van Blitterswijk, C. A. and de Boer, J. (2012) Forskolin enhances in vivo bone formation by human mesenchymal stromal cells. Tissue Eng Part A. 18, 558-567

2. Roszczyk, M. and Juszczak, M. (2014) Forskolin-stimulated vasopressin and oxytocin release from the rat hypothalamo-neurohypophysial system in vitro is inhibited by melatonin. Endokrynol Pol. 65, 125-131

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