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Streptavidin – FITC

Catalog No.
K1081
Used in fluorescent detection of biotin conjugated antibodies, proteins or other molecules
Grouped product items
SizePriceStock Qty
500ug
$80.00
In stock
1mg
$120.00
In stock

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Background

Streptavidin is a 52,800 dalton homotetramer protein. It has an extraordinarily high affinity for biotin, one molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin- FITC is used in fluorescent staining of biotinylated antibodies, proteins, nucleic acids or other molecules in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), in situ hybridization (ISH), or flow cytometry. FITC is excited maximally at 488 nm, emits maximally at ~520 nm.

Chemical Properties

Physical AppearanceSolution. Streptavidin-FITC is supplied in phosphate buffered saline (PBS), pH 7.6, containing 1% BSA, 0.1% sodium azide.
StorageStore at 2-8°C. Light exposure should be avoided. Do not freeze.
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Concentration

0.5 mg/ml
Recommended Dilution:
1:20 to 1:50 dilution with PBS containing 10% normal goat serum. Working dilution for specific application should be determined by individual investigator to obtain the best conditions.

Quality Control

Quality Control & MSDS

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Related Biological Data

Streptavidin – FITC