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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HPF(Hydroxyphenyl fluorescein) is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence.HPF reacts with intracellular peroxynitrite through hydroxyl radicals, generating intense green fluorescence. It offers high specificity and stability: oxidation occurs only in the presence of highly reactive oxygen species (hROS) such as hydroxyl radicals, peroxynitrite, and hROS generated by peroxidase/H₂O₂ systems. HPF does not react with hypochlorite ions, nitric oxide, hydrogen peroxide, superoxide, or other oxidants. Upon oxidation, HPF converts to the highly fluorescent molecule fluorescein, with excitation/emission peaks at 490/515 nm, making the direct detection of highly reactive biological radicals simple and intuitive. HPF can be used in fluorescence microscopy, high-throughput imaging systems, fluorescence plate readers, or flow cytometry.
