BODIPY 581/591 C11

BODIPY 581/591 C11 (CAS 217075-36-0) is a cell-permeable, ratiometric fluorescent probe used to detect lipid peroxidation and evaluate antioxidant capacity in biological membranes or live cells. Under reduced conditions, it displays red fluorescence at excitation/emission wavelengths of approximately 581/591 nm. Upon oxidation of its polyunsaturated butadienyl segment by reactive oxygen species (e.g., hydroxyl radicals, peroxynitrite), emission shifts from 590 nm to 510 nm, resulting in green fluorescence (excitation/emission around 488/510 nm). Its specificity and photostability make BODIPY 581/591 C11 suitable for quantifying lipid oxidative stress in biomedical research.

BODIPY 581/591 C11 is a cell-permeable, ratiometric fluorescent probe that can indicate lipid peroxidation levels and antioxidant properties within model membrane systems or living cells. Under normal circumstances, BODIPY 581/591 C11 is in a reduced state, and its excitation and emission wavelengths are 581/591 nm, which is red fluorescence; and when the polyunsaturated butadienyl moiety of BODIPY 581/591 C11 is oxidized, its emission wavelength shifts from 590 nm to 510 nm, and the excitation wavelength and emission wavelength are 488/510 nm, which is green fluorescence. BODIPY 581/591 C11 has good photostability, high quantum yield, and is sensitive to oxygen radicals and peroxynitrite, but not to superoxide, nitric oxide and hydrogen peroxide.