Protein A/G Magnetic Co-IP/IP Kit
Protein A/G Magnetic Co-IP/IP Kit comprises recombinant Protein A/G covalently immobilized onto nano-sized magnetic beads, enabling specific binding through antibody Fc regions. Protein A/G binds various mammalian immunoglobulins, facilitating targeted immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) of protein complexes for downstream analyses including SDS-PAGE and mass spectrometry. The kit can be applied to studying protein-protein interactions, isolating immune complexes from cell lysates, serum, or culture supernatants, as well as antibody purification. Magnetic separation simplifies handling, shortens incubation periods, and minimizes protein degradation risks.
- 1. Xian-Pei Zhou, Qi-Wei Li, et al. "PML Regulated HIF1AN Ubiquitination and Activated PI3K/AKT Pathway to Promote Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation." Int J Stem Cells. 2025 May 30;18(2):146-157 PMID: 40059075
- 2. Rongjun Xiao, Qingsong Wang, Jun Peng. "BMSCs-derived exosomal Egr2 inhibited OGD/R-induced neuronal cell injury through the RNF8/DAPK1 axis in ischemic stroke." Exp Brain Res. 2025 Jun 26;243(7):181 PMID: 40571783
| Components | K1309-10 T | K1309-50 T |
| Cell Lysis Buffer | 5 mL | 25 mL |
| Protease Inhibitor Cocktail (EDTA-Free,100X in DMSO) | 50 μL | 250 μL |
| 10X TBS | 5 mL | 30 mL |
| Neutralization Buffer | 100 μL | 500 μL |
| Acid Elution Buffer | 1 mL | 5 mL |
| Protein A/G beads | 200 μL | 1 mL |
| 5X Protein Loading Buffer (Reducing) | 200 μL | 1 mL |
Store Protease Inhibitor Cocktail (EDTA-Free,100X in DMSO) and 5X Protein Loading Buffer (Reducing) at -20°C and the rest of components at 4°C for 12 months. | ||