Clodronate Liposomes

To investigate the function of macrophages in a complex in vivo environment, depletion experiment can be utilized. Clodronate liposomes are an effective and versatile way to deplete macrophages in vivo. Clodronate liposomes function on the principle of delivering a potent macrophage-depleting agent, clodronate, encapsulated within a lipid bilayer. When administered in vivo, these liposomes are specifically taken up by macrophages through a process known as phagocytosis. Once internalized, the liposomes release clodronate into the macrophage, ultimately resulting in cell death through apoptosis.

Clodronate liposomes can allow selective depletion from tissues of interest and can be used on transgenic mice. This reagent supports a variety of administration methods, including intravenous injection, intraperitoneal injection, subcutaneous injection, intranasal injection, and direct injection into the testicles. The injection dose varies according to the body weight of the mouse, the frequency of injection, the mode of administration, and the experimental needs. This reagent is recommended for use in conjunction with PBS Liposomes (Cat. No. K2722), which can be used as a blank control for this reagent.

Figure 1: Mouse were treated with PBS Liposomes (Cat. Number: K2722) or Clodronate Liposomes (Cat. Number: K2721). Tissue was taken after death for the mouse macrophage maker F4/80 staining. The result showed that Clodronate Liposomes could significantly deplete macrophages