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Auranofin

Catalog No.
B7687
thioredoxin reductase (TrxR) inhibitor
Grouped product items
SizePriceStock Qty
10mg
$68.00
In stock
50mg
$115.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Auranofin (CAS: 34031-32-8) is a small molecule inhibitor targeting thioredoxin reductase (TrxR), a flavoenzyme responsible for channeling electrons from NADPH to thioredoxin, crucial in various physiological processes including apoptosis and oxidative stress response. Auranofin inhibits TrxR with an IC50 of approximately 88 nM. In assays against Helicobacter pylori, this inhibitor suppressed bacterial growth at concentrations around 1.2 μM. Additionally, treatment of murine 4T1 and EMT6 tumor cells with Auranofin (3–10 μM) enhanced their sensitivity to radiation treatment, underscoring its potential for biomedical research in oncology and antimicrobial studies.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at RT
M.Wt678.48
Cas No.34031-32-8
FormulaC20H34AuO9PS
Solubility≥67.8 mg/mL in DMSO; insoluble in H2O; ≥31.6 mg/mL in EtOH
SDFDownload SDF
Canonical SMILESO=C(C)OC1C(OC(C)=O)C(OC(C)=O)C(COC(C)=O)OC1[S-].CCP(CC)CC.[Au+]
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

PC3 human prostate cancer cells

Reaction Conditions

3.125 ~ 100 μM auranofin for 24 h incubation

Applications

Treatment with auranofin significantly inhibited cell viability with an IC50 value of 2.5 μM after 24 h. Auranofin treatment (1.25 ~ 10 μM) activated caspase-3 and caspase-8 in a concentration-dependent manner and decreased the levels of mitochondrial anti-apoptotic factors, such as Bcl-2 and Bcl-xL.

Animal experiment:[2]

Animal models

4T1 tumor-bearing mice

Dosage form

3 mg/kg

Administrated subcutaneously from day 6 to 10 and day 13 to 17

Applications

In 4T1 tumor-bearing mice, auranofin combined with buthionine sulfoximine, subcutaneously administrated at the concentrations of 3 and 25 mg/kg, respectively, enhanced tumor radioresponse, resulting in a tumor growth delay of 13 days, and thereby significantly increased the medium survival rate, while neither of these pharmaceuticals were effective when administered on their own.

Note

The technical data provided above is for reference only.

References:

1. Park N, Chun YJ. Auranofin promotes mitochondrial apoptosis by inducing annexin A5 expression and translocation in human prostate cancer cells. The Journal of Toxicology and Environmental Health, Part A: Current Issues, 2014, 77(22-24): 1467-1476.

2. Wang H, Bouzakoura S, de Mey S, et al. Auranofin radiosensitizes tumor cells through targeting thioredoxin reductase and resulting overproduction of reactive oxygen species. Oncotarget, 2017, 8(22): 35728-35742.

Quality Control

Chemical structure

Auranofin

Related Biological Data

Auranofin