Quality Control & DataSheet
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Components and storage
|Complete Kit||200 rxns||500 rxns|
|Lysis buffer||20 mL||50 mL|
|Balance buffer||20 mL||50 mL|
|2×PCR Master Mix (With Dye)||1 mL x 2||1 mL x 5|
|Proteinase K||200 μl||500 μl|
1. Lysis buffer and Balance buffer should be stored at 4°C.
2. 2 x PCR Master Mix could be stored at -20°C for 2 years.
3. Store the unopened Protease K at -20 to -70 °C. Avoid repeated freeze/thaw cycles. Upon first use, it is recommended to aliquot sample into single use portions. The Proteinase K solution should be stored at -20°C and could be stored at 4°C for a short time.
The Genotyping Kit for target alleles of insects, tissues, fishes and cells provides convenience in faster preparation and superior PCR amplification. Lysis buffer and balance buffer could digest tissues or cells rapidly to release unbroken genomic DNA, and it could be used as PCR template directly without extraction. There is no need for overnight digestion, Phenol/Chloroform extraction, toilsome manual purification or the utilization of expensive DNA spin columns. By using our kit, you can substantially shorten your time for digestion. Furthermore, the 2×PCR Master Mix (With Dye) will guarantee a robust and accurate amplification consequence. This Master Mix contains dye which means it can be directly electrophoresed after the amplification without the need to add a loading buffer. In addition, our genotyping Kit mitigates the risk of sample cross-contamination occurring in the PCR reaction by conducting the DNA extraction in one single tube.