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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Aminoallyl-UTP is used for Nucleic Acid Sequence Based Amplification (NASBA), Microarray based hybridization detection, Eberwine mRNA amplification and RNA-labeling. It is enzymatically incorporated into RNA during the NASBA reaction and Eberwine mRNA amplification. The resulting amine-functionalized RNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microarray based hybridization detection.
References:[1] Scheler et al. (2009) Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications. BMC Biotechnol. 9:45.[2] Hoen et al. (2003) Fluorescent labelling of cRNA for microarray applications. Nucleic Acids Res. 31 (5):e20.
