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2×Taq PCR Master Mix(with dye)

In stock
Catalog No.
K1034
 
Grouped product items
SizePriceStock Qty
1ml
$20.00
In stock
5x1ml
$60.00
In stock
20x1ml
$200.00
In stock
50x1ml
$400.00
In stock
100x1ml
$640.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Description

Our product 2×Taq PCR Master Mix is a ready-to-use 2× premix solution containing Taq DNA Polymerase, dNTPs, an already optimized buffer system and dye. Taq DNA polymerase synthesizes DNA under appropriate conditions from single-stranded templates in the presence of the gene-specific primers and dNTPs. It’s a recombinant product extracted from E.coli with thermu aquaticus DNA polymerase gene expressed.

Taq DNA Polymerase possesses the capability of 5'→3' DNA polymerase and weak 5'→3' exonuclease, yet, no potential of 3'→5' exonuclease which means there will appear an dA overhang at the 3’ end. Using this characteristic, it can be used in TA cloning. In PCR reaction, elongation rate of Taq DNA Polymerase is about 1-2 kb/min depending on the complexity of the gene. For most templates, using 1kb/min.

The reaction system is ready to use by adding primers, templates and ddH2O, simplifying the experimental steps, reducing personal error and improving the repeatability of the results.

This Master Mix contains dye which means it can be directly electrophoresed after the amplification without the need to add a loading buffer. If needed to subject the PCR product to agarose gel electrophoresis, our product SYBR Safe DNA Gel Stain (Cat: A8743) is available.

Quality Control

Quality Control & DataSheet

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Related Biological Data

2×Taq PCR Master Mix(with dye)

Related Biological Data

2×Taq PCR Master Mix(with dye)
 

Components and Storage

Component Size
2×Taq PCR Master Mix(with dye)
1ml
5×1ml
20×1ml
50×1ml
100×1ml
Store the 2Xmaster mix at -20°C.

Specialty

Ideal product for general PCR or other experiments
Quick and simply ready-to-use PCR mix
Less cost per unit
Containing dye already