Western Blot | One-Stop Reagent Supply
Overview
Western Blot Reagents Overview
| Steps | Subcategories | Features |
|---|---|---|
| Sample Preparation |
Preserves protein integrity and post-translational modifications
Optimized for different sample types (mammalian, bacterial, plant, etc.)
Ready-to-use formats for convenience
|
|
| Protein Quantification |
Accurate and sensitive protein concentration measurement
Compatible with various lysis buffers
Wide dynamic ranges
|
|
| Protein Electrophoresis |
Wide range of protein loading buffers
Protein markers for accurate molecular weight determination
Complete gel preparation kits for SDS-PAGE and Native-PAGE
Comprehensive selection of electrophoresis running buffers
|
|
| Transfer | Transfer Buffers |
Efficient protein transfer from gel to membrane
Compatible with both wet and semi-dry transfer systems
|
| Blocking & Antibody Incubation |
Low background and high signal-to-noise ratio
Rich and diverse portfolio of primary antibodies (monoclonal & polyclonal; covering human, mouse,
rat, and other species)
Wide range of secondary antibody conjugates available (HRP, AP, Biotin, FITC, Cy3, Cy5, HyperFluor™ 488
series)
Broad range of blocking buffers (protein-based and rapid Protein-Free formulations)
Specialized antibody dilution and wash buffers
|
|
| Protein Detection |
High sensitivity detection
Multiple detection platforms supported
Long signal duration for ECL substrates
|
|
| Antibody Stripping | Stripping Buffers |
Allows re-probing of membranes with multiple antibodies
Preserves membrane integrity
|
| Supplementary Reagents |
General Buffers
Buffer Components
Protein Stains & Indicators
|
High purity reagents
Consistent quality between batches
|
Sample Preparation
Effective sample preparation prevents protein degradation and preserves post-translational modifications. Our inhibitor cocktails and lysis buffers are optimized for mammalian, bacterial, fungal, and plant samples, with subcellular fractionation kits for organelle-specific analysis. Ready-to-use formats ensure reproducibility.
Inhibitor Cocktails
Our inhibitor cocktails deliver broad-spectrum protection across protease, phosphatase, and deacetylase targets. Choose from water-soluble or DMSO formulations, optimized for specific sample types. Preserve native protein abundance and PTMs for WB, IP/Co-IP, ELISA, and purification.
| Protease Inhibitor Cocktail | Phosphatase Inhibitor Cocktail |
|---|---|
|
K1007
 K1008 For secreted
protein samples
K1009 For fungi
or yeast samples
K1010 For
His-tagged protein purification
K1011 For plant
samples
K1019 K1007 + EDTA
(supplied separately)
K1024 For bacterial
cell, Includes EDTA tube
K4001
K4002
K4003
Water-soluble/EDTA pre-mixed
K4007 K4003 without
EDTA
A2587 PMSF, Classic serine protease inhibitor
|
K1012 Ser/Thr &
alkaline phosphatases
K1013 Tyrosine, acid
& alkaline phosphatases
K1014 Ser/Thr &
alkaline phosphatases
K1015
K4004
|
| Protease & Phosphatase Cocktail | Deacetylase Inhibitor Cocktail |
|
K4005 EDTA (supplied
separately), 2 tubes
K4006
|
K1017 Maintains protein acetylation status |
Validation
Lysis Buffers & Protein Extraction Kits
Our lysis buffers are formulated for efficient cell lysis and protein extraction from various sample types, with different stringency levels to preserve protein function and interactions.
| Lysis Buffers | Protein Extraction Kits |
|---|---|
|
K1020
K1120 K1020 without
inhibitors
K1121 RIPA (medium), includes inhibitors
K1122 RIPA (Weak), includes inhibitors
K1123 For WB/IP, includes inhibitors
K1124 K1123 without
inhibitors
K1127 1% NP-40, includes inhibitors
K1128 1% SDS, includes inhibitors
K1125 Plant RIPA (Strong), includes
inhibitors
K1126 Plant tissues/cells, WB
& IP
|
K1137 Nucleic Fluid Extraction
Isolation Kit
K1138 Mitochondria Isolation
Kit
K4061
K4062 Active Protein
Extraction (Insect)
K4063 Active Protein
Extraction (Bacteria)
|
Validation
Protein Quantification
Accurate protein quantification is essential for loading equal amounts of protein in Western Blot experiments. We offer a range of quantification methods to suit different sample types and concentrations.
| Catalog No. | Products | Detection | Features |
|---|---|---|---|
| K4101 | BCA Protein Assay Kit | OD562 |
Classic BCA assay
50-2000 μg/mL range
|
| K4102 |
Micro BCA Protein Assay Kit | OD562 |
For low protein concentrations
0.5-20 μg/mL range
|
| K4103 | Bradford Protein Assay Kit | OD595 | Classic Bradford assay |
| K4104 |
Detergent Compatible Bradford Protein Assay Kit | OD595 | Bradford assay with detergent compatibility |
| K4105 | Bovine Serum Albumin Standard (5 mg/mL) | - | Protein standard (BSA, 5 mg/mL) |
Validation
Protein Electrophoresis
Protein electrophoresis separates proteins based on their molecular weight, allowing for size-based analysis. We offer a complete range of reagents for both SDS-PAGE and native PAGE.
| Loading Buffers | Protein Markers |
|---|---|
|
K1164
K1165 5X (non-reducing)
K1166 2X (reducing)
K1167 2X (non-reducing)
K1168 50X reducing buffer
|
F4005
F4006 Unstained, EDTA included,
10-225 kDa
|
| Running Buffers | Gel Preparation Kit |
|
K1196 Tris-Glycine, Liquid (10X), Classic
K1197 Tris-Glycine, Liquid (5X), Classic
H2006 MOPS, Powder, Bis-Tris
system
H2008 MES, Powder, Bis-Tris
system
|
K4141
Native-PAGE (pI>7), Gel kit/loading/running buffers
K4142
Native-PAGE (pI≤7), Gel kit/loading/running buffers
K4131 SDS-PAGE (Tris-Gly, 6%),
SDS-free
K4132 SDS-PAGE (Tris-Gly, 8%),
SDS-free
K4133
K4134 SDS-PAGE (Tris-Gly,
12%), SDS-free
K4135 SDS-PAGE (Tris-Gly,
15%), SDS-free
K4136 SDS-PAGE (Tricine),
SDS-free
|
Validation
Transfer
Optimized transfer buffers for efficient protein blotting from polyacrylamide gels to membranes. Available in liquid (10X) and powder formats, suitable for both wet/tank and semi-dry transfer systems using Tris-Glycine buffer systems.
| Catalog No. | Products | Format | Features |
|---|---|---|---|
| K1198 |
10X Transfer Buffer | Liquid (10X) | Classic transfer buffer (Tris-Gly system) |
| H2023 | Transfer Buffer (Semi Dry, powder) | Powder | Transfer buffer for semi-dry electroblotting (Tris-Gly system) |
Blocking & Antibody Incubation
Blocking prevents non-specific antibody binding, while antibody incubation allows specific detection of target proteins.
| Blocking Buffers | Antibodies |
|---|---|
|
B7773 BSA, powder
K4106
K4118 For WB, Casein in PBS,
Liquid
K4119 For WB, Casein in TBS,
Liquid
K4122 Protein-Free in PBS, Liquid,
5-15 min
K4123 Protein-Free in PBSTw,
Liquid, 5-15 min
K4124 Protein-Free in PBSTx,
Liquid, 5-15 min
K4125 Protein-Free in TBS, Liquid,
5-15 min
K4126 Protein-Free in TBSTw,
Liquid, 5-15 min
K4127 Protein-Free in TBSTx,
Liquid, 5-15 min
K4128 Protein-Free, Liquid (10X)
for various buffers
K4129
K4130
Protein-Free, for IF/IHC, Liquid, 5-15 min
H2022 Fish gelatin in TBS-T, powder
|
Loading Control Antibodies: e.g. GAPDH, β‑actin.
Total Protein Antibodies: e.g. NF‑κB, p38 MAPK.
Phospho‑Protein Antibodies: e.g. pNF‑κB, pp38.
Fluorescent Conjugates:
FITC/ Cy3/ Cy5
HyperFluor™ 405/488/555/594/647
|
| Antibody Dilution Buffer | Wash Buffer |
|
K1200 For Primary Antibody,
BSA/Triton X-100
K4115 For WB Secondary,
BSA/detergents
K4140
K4662
K4663 For IF/IHC/IC
Primary, Protein-free, High S/N
K4664 For IHC
Secondary, Protein-free, High S/N
* High S/N: High signal-to-noise
|
K4116 WB Wash Buffer, 1X ready-to-use
K4117 WB Wash Buffer, 10X concentrate
|
Validation
Protein Detection
Protein detection visualizes the bound antibody-target protein complex. We offer both chemiluminescent and colorimetric detection methods.
Chemiluminescent Detection (ECL Chemiluminescent Substrates)
Enhanced chemiluminescence (ECL) substrates for sensitive detection of HRP-labeled antibodies in Western blotting. Available in multiple sensitivity levels (standard, enhanced, hypersensitive, ultrasensitive, and supersensitive) to accommodate various target abundance and detection requirements.
|
Standard
|
Enhanced
|
Hypersensitive
|
Ultrasensitive
|
Ultrasensitve (SuperSignal)
|
|
|---|---|---|---|---|---|
| Sensitivity | Picogram (pg) | Low picogram | Low picogram to femtogram （fg） | Femtogram (fg) | Femtogram (fg) |
| Signal Duration | ≤ 2 hours | ≤ 5 hours | 6 - 8 hours | ≤ 8 hours | ≤ 16 hours |
| Recommended Use | Routine WB, high-abundance targets, cost-effective | Need brighter signal & longer detection window | 1st Choice for low-abundance proteins, pg-fg sensitivity, and budget friendly | Fg sensitivity, very low-abundance / precious samples | Fg sensitivity, very low-abundance / precious samples, overnight imaging |
Validation
Colorimetric Detection (Chromogenic substrates)
Chromogenic substrates for non-chemiluminescent protein detection. Includes Coomassie Blue-based staining for in-gel protein visualization, BCIP/NBT for alkaline phosphatase (AP) detection, DAB for HRP-based staining, and TMB for blotting applications.
| Catalog No. | Products | Color | Features |
|---|---|---|---|
| B8226 |
InstaBlue Protein Stain Solution | Blue | Total protein stain for SDS-PAGE gels |
| K4151 | BCIP/NBT Alkaline Phosphatase Color Development Kit | Blue-purple | AP substrate; for WB, IHC |
| K4152 | DAB Horseradish Peroxidase Color Development Kit | Brown | HRP substrate; for WB, IHC |
| K1130 | TMB substrate solution for Blotting | Dark blue | HRP substrate; for WB, IHC |
Validation
Stripping Buffer
Western stripping buffers remove bound antibodies from the membrane, allowing it to be re-probed with different antibodies.
| Catalog No. | Products | Features | When to Use |
|---|---|---|---|
| K4111 |
Western Stripping Buffer (Acidic) | Acidic, mild condition, ~40-80 min protocol | Routine stripping, best for preserving protein |
| K4112 |
Western Stripping Buffer (Neutral) | Neutral, non-corrosive, safer handling, ~20-30 min protocol | Antigen is acid-sensitive, safer handling |
| K4113 | Western Stripping Buffer (Weak alkali) | Weak alkaline, balanced efficiency & safety, ~20-30 min protocol | Balanced efficiency & safety, when acidic is insufficient |
| K4114 | Western Stripping Buffer (Strongly alkali) | Strong alkaline, most effective, higher protein loss risk, ~15-20 min protocol | Robust, high-affinity antibodies, other buffers ineffective |
Supplementary Reagents
Ready-to-use and powder-formulated common laboratory buffers including PBS, PBST, TBS, TBST and individual buffer components.
| General Buffers | Buffer Components and Protein Stains & Indicators |
|---|---|
|
H2021 TBS, powder, 1L/pack
K1199
H2020 TBST, powder, 1L/pack
K2818
H2018 PBS, powder, 1L/pack
|
B7303 HEPES Sodium salt
B1861 Tris (Trometamol)
B7772 Bis-Tris
C5579 Coomassie G-250
B7774 Bromophenol Blue
|
Validation
