Western Blocking Buffer (Casein in PBS)

Western Blocking Buffer (Casein in PBS) is a milk protein purified by the Hammarsten method, used for blocking excess binding sites in ELISA, Western blot, immunohistochemistry, and other immunochemical applications. Western Blocking Buffer (Casein in PBS) is formulated in phosphate-buffered saline (PBS) or Tris-buffered saline (TBS) containing 1% (w/v) casein. Compared to serum- or milk-based blocking buffers, this single purified protein offers fewer chances of cross-reactivity with assay components. Western Blocking Buffer (Casein in PBS) is an excellent alternative when high background or antigen/antibody masking occurs with non-fat milk blockers.