Aminoallyl-dUTP
Catalog No.
B7950
Labeling of DNA/cDNA via PCR and functions as a reactive site for subsequent labeling by reactive fluorescent probes.
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Aminoallyl-dUTP (CAS 116840-18-7) is a modified deoxynucleotide designed for indirect, covalent labeling of DNA or cDNA in molecular biology research. Structurally analogous to natural dTTP, it incorporates into DNA via enzymatic reactions including PCR (Taq polymerase), nick translation, primer extension (Klenow exo-), terminal labeling (TdT), and reverse transcription (MMLV RT). The introduced primary amine moiety enables subsequent conjugation to NHS-activated labels such as fluorophores for fluorescence microscopy or biotin for affinity-based purification and detection strategies including in situ hybridization.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 523.22(free acid) |
| Cas No. | 116840-18-7 |
| Formula | C12H20N3O14P3(free acid) |
| Synonyms | 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate,aa-dutp ,aa dutp,5-AA-dUTP,5-Aminoallyl-2'-deoxyuridine-5'-Triphosphate |
| Solubility | Soluble in sterile water |
| Chemical Name | ((2R,3S,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate |
| SDF | Download SDF |
| Canonical SMILES | O=C(N1)N([C@@H]2O[C@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[C@@H](O)C2)C=C(/C=C/CN)C1=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Protocol
Chemical structure
